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low input library prep kit v2  (TaKaRa)


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    Structured Review

    TaKaRa low input library prep kit v2
    Low Input Library Prep Kit V2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 3231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+input+library+prep+kit+v2/SMART-Seq+v4+Ultra+Low+Input+RNA+Kit+for+Sequencing/bio_rxiv__64898__2026__01__02__697403-182-24-30
    Average 98 stars, based on 3231 article reviews
    low input library prep kit v2 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Purification:

    Article Title: Hexanucleotide repeat expansion in SCA36 reduces the expression of genes involved in ribosome biosynthesis and protein translation.
    Article Snippet: Hereditary spinocerebellar ataxia (SCA) is a group of clinically and genetically heterogeneous inherited disorders characterized by slowly progressive cerebellar ataxia.. We ascertained a Japanese pedigree with autosomal dominant SCA comprising four family members, including two patients.. We identified a GGCCTG repeat expansion of intron 1 in the NOP56 gene by Southern blotting, resulting in a molecular diagnosis of SCA36.

    Sequencing:

    Article Title: Hexanucleotide repeat expansion in SCA36 reduces the expression of genes involved in ribosome biosynthesis and protein translation.
    Article Snippet: Hereditary spinocerebellar ataxia (SCA) is a group of clinically and genetically heterogeneous inherited disorders characterized by slowly progressive cerebellar ataxia.. We ascertained a Japanese pedigree with autosomal dominant SCA comprising four family members, including two patients.. We identified a GGCCTG repeat expansion of intron 1 in the NOP56 gene by Southern blotting, resulting in a molecular diagnosis of SCA36.

    Article Title: Transcription factors underlying photoreceptor diversity
    Article Snippet: .. We used the SMART-seq v4 ultra-low input RNA kit for sequencing (Takara #634897) using the manufacturer’s instructions for single-cell samples, followed by the Low Input Library Prep Kit v2 (Takara #634899). .. For sequencing, we pooled up to 12 samples (with different barcodes) in one lane of a flow cell (Illumina HiSeq 2500) and used a 150 bp paired-end read configuration.

    Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity.
    Article Snippet: Total RNA was extracted from bone marrow sorted samples (Lineage negative, CD117+, SCA-1+) using RNeasy Plus Micro Kit (Qiagen #74034). .. Libraries were generated with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing and Low Input Library Prep Kit v2 (Takara Bio # 634899). .. Libraries were sequenced on an Illumina HiSeq 4000 sequencer.

    RNA sequencing:

    Article Title: Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation
    Article Snippet: For accessions GSE126428 and GSE126625, RNA was amplified and prepared for RNA sequencing (RNA-Seq) using the SMART-Seq® v4 Ultra® Low Input RNA kit (Clontech TaKaRa Bio). .. The RNA-Seq library was prepared with the Low Input Library Prep Kit v2 (Clontech TaKaRa Bio) to construct index-tagged cDNA. .. Libraries were sequenced on the Illumina HiSeq 2500 (Illumina) following the standard sequencing protocol with the TruSeq SBS Kit v4 and the following software versions: HCS 2.2.58, RTA 1.18.64.

    Article Title: Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation.
    Article Snippet: For accessions GSE126428 andGSE126625, RNAwas amplified and prepared for RNA sequencing (RNA-Seq) using the SMART-Seq® v4 Ultra® Low Input RNA kit (Clontech TaKaRa Bio). .. The RNA-Seq library was prepared with the Low Input Library Prep Kit v2 (Clontech TaKaRa Bio) to construct index-tagged cDNA. .. Libraries were sequenced on the Illumina HiSeq 2500 (Illumina) following the standard sequencing protocol with the TruSeq SBS Kit v4 and the following software versions: HCS 2.2.58, RTA 1.18.64.

    Article Title: A prelimbic molecular clock of protein synthesis for memory persistence
    Article Snippet: The integrity of the RNA was assessed with a High Sensitivity ScreenTape (Agilent Catalog #5067-5579), and only samples with an RIN greater than 9 were selected for further analysis. .. RNA-Seq libraries were prepared from 50–200 pg of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Illumina Sequencing, with the Low Input Library Prep kit v2 (Clontech, Catalog # 634890 and 634899, respectively). .. Libraries were sequenced using an Illumina HiSeq 2500 instrument with a paired-end 50 protocol.

    Construct:

    Article Title: Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation
    Article Snippet: For accessions GSE126428 and GSE126625, RNA was amplified and prepared for RNA sequencing (RNA-Seq) using the SMART-Seq® v4 Ultra® Low Input RNA kit (Clontech TaKaRa Bio). .. The RNA-Seq library was prepared with the Low Input Library Prep Kit v2 (Clontech TaKaRa Bio) to construct index-tagged cDNA. .. Libraries were sequenced on the Illumina HiSeq 2500 (Illumina) following the standard sequencing protocol with the TruSeq SBS Kit v4 and the following software versions: HCS 2.2.58, RTA 1.18.64.

    Article Title: Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation.
    Article Snippet: For accessions GSE126428 andGSE126625, RNAwas amplified and prepared for RNA sequencing (RNA-Seq) using the SMART-Seq® v4 Ultra® Low Input RNA kit (Clontech TaKaRa Bio). .. The RNA-Seq library was prepared with the Low Input Library Prep Kit v2 (Clontech TaKaRa Bio) to construct index-tagged cDNA. .. Libraries were sequenced on the Illumina HiSeq 2500 (Illumina) following the standard sequencing protocol with the TruSeq SBS Kit v4 and the following software versions: HCS 2.2.58, RTA 1.18.64.

    Single Cell:

    Article Title: Transcription factors underlying photoreceptor diversity
    Article Snippet: .. We used the SMART-seq v4 ultra-low input RNA kit for sequencing (Takara #634897) using the manufacturer’s instructions for single-cell samples, followed by the Low Input Library Prep Kit v2 (Takara #634899). .. For sequencing, we pooled up to 12 samples (with different barcodes) in one lane of a flow cell (Illumina HiSeq 2500) and used a 150 bp paired-end read configuration.

    Generated:

    Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity.
    Article Snippet: Total RNA was extracted from bone marrow sorted samples (Lineage negative, CD117+, SCA-1+) using RNeasy Plus Micro Kit (Qiagen #74034). .. Libraries were generated with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing and Low Input Library Prep Kit v2 (Takara Bio # 634899). .. Libraries were sequenced on an Illumina HiSeq 4000 sequencer.



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